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UV Absorption Spectroscopy and its Applications

This module is on Ultraviolet (UV) Visible absorption spectroscopy: a spectro-analytical procedure for the quantitative determination of compounds containing chromophores that absorb UV.

  • Basics of spectroscopy and electromagnetic spectrum

  • Principles of UV Visible spectroscopy and role of chromophores

  • Beer Lambert’s law and its applications

  • Factors affective absorbance

  • Components of a UV Visible spectrophotometer

  • Calibration and operation of UV-Vis spectrophotometer

  • Procedure for identification test

  • Procedure for concentration measurement

  • Best practices and precautions while handling the UV Visible spectrophotometer

Why it matters

UV spectroscopy is the most used method in identification and quantification of biological samples\, such as DNA\, RNA\, and proteins with aromatic amino acids with applications in enzyme kinetics\, determination of equilibrium constants\, drug dissolution\, conformation changes in proteins\, and DNA melting studies. It is crucial that analysts understand the science behind UV spectroscopy in order to conduct these experiments accurately.

Who is it for

Personnel in production\, analytical quality\, including quality control and quality assurance who use this technique should complete this module.

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