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A Practical Guide to Sample Preparation Techniques - Part 2

Part 2 of this two-part module covers the basics of sample preparation focusing on pipetting, filtration, centrifugation and vial filling.

  • Principle of pipetting, graduated pipettes, and pipetting parameters

  • Selection of the right pipette and reading from a pipette (meniscus)

  • Pipetting steps and critical precautions

  • Introduction to filtration and types of filters

  • Syringe filters and steps to filtration

  • Critical parameters and precautions

  • Introduction to centrifugation and types o centrifuges

  • Difference between centrifugation and filtration

  • Centrifugation steps and the critical parameters

  • Vial filling using syringe filtration

  • Vial labeling and crimping

  • Typical do’s and don’ts for all techniques

Why it matters

Proper sample preparation is critical to ensure that evaluation of batch quality is accurately performed\, and to ensure reproducible results. Pipetting is used to measure and transfer an accurate volume of liquid. Centrifugation and filtration are different ways of removing solids from liquids and ensure that the samples used for analysis are particle free.. Understanding and training on these techniques will ensure exact sample preparation and accurate results.

Who is it for

This module is for employees in production quality control and quality assurance who prepare samples for in-process or final testing.

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