UV Absorption Spectroscopy and its Applications
This module is on Ultraviolet (UV) Visible absorption spectroscopy: a spectro-analytical procedure for the quantitative determination of compounds containing chromophores that absorb UV.
Basics of spectroscopy and electromagnetic spectrum
Principles of UV Visible spectroscopy and role of chromophores
Beer Lambert’s law and its applications
Factors affective absorbance
Components of a UV Visible spectrophotometer
Calibration and operation of UV-Vis spectrophotometer
Procedure for identification test
Procedure for concentration measurement
Best practices and precautions while handling the UV Visible spectrophotometer
Why it matters
UV spectroscopy is the most used method in identification and quantification of biological samples such as DNA, RNA, and proteins with aromatic amino acids. UV has applications in enzyme kinetics, determination of equilibrium constants, drug dissolution, conformation changes in proteins, and DNA melting studies. It is crucial that analysts understand the science behind UV spectroscopy in order to conduct these experiments accurately.
Who is it for
Personnel in production, analytical quality, including quality control and quality assurance who use this technique should complete this module.