Practical Guide to Sample Preparation Techniques - Part 1
Part 1 of this two-part module covers the basics of sample preparation focusing on weighing, crushing and grinding, sonication, shaking, dilutions and glassware handling
Types of weighing balances, steps to weighing and typical precautions
Grinding tools such as porcelain, agate, stainless steel or automated grinder
Parameters when selecting mortar and pestle
Sonication and different types of sonicators including probe type and ultrasonic bath.
Steps to sonication and shaking
Introduction to dilution and volumetric flasks
Glassware handling including selection of glassware
Maintenance and cleaning of glassware as per USP
Proper handling of glassware with typical do’s and don’ts
Why it matters
Proper sample preparation is critical to ensure that evaluation of batch quality is accurately performed, and to ensure reproducible results. Grinding and crushing followed by weighing, are the oldest ways of preparing samples for a composite assay of pharmaceutical tablets. Similarly, sonication can be used for degassing, particle size reduction and agitation. Knowledge of these techniques, and their various applications is crucial at pharmaceutical enterprises.
Who is it for
This module is for employees in production quality control and quality assurance who prepare samples for in-process or final testing.