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HPLC and its Applications

HPLC and its Applications

This GMP course is designed to explain the fundamental concepts of High-Performance Liquid Chromatography (HPLC), a common   technique used to separate  individual compounds in a mixture.​


  • What is Chromatography ​

  • Different separation modes​

  • What is High Performance liquid chromatography ​

  • Components of a typical HPLC instrument
    • Column​

    • Detectors​

    • Pumps​

    • Autosamplers​

  • Understanding system suitability parameters​
  • Importance of a blank and standards​
  • Data Analysis​
  • Maintaining Data Integrity​
  • Why it matters

    In the pharmaceutical industry, HPLC is a ubiquitous process used to test the quality of intermediate and final products, It is also used to detect impurities or do identification test for raw materials. HPLC is fast and efficient, provides reproducible results, and can be adapted for different types of products easily by simple changes in column, detector, and methods​

  • Who is it for​ ​

    This course is designed as basic analytical training for all quality control personnel involved in analysis of drug substance, drug product, raw materials and in-process samples. It is also valuable for people in manufacturing and quality assurance.​

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