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Basics of Chromatography

Basics of Chromatography

This  eLearning  module is to understand, the basics of Chromatography, including the most commonly used separation techniques, such as high performance liquid chromatography and gas chromatography, and their basic instrumentation.​


  • Review the terms related to chromatography including chromatogram, retention time, blank, etc.​

  • Understand the role of mobile and stationary phases in separation of molecules during analysis.​

  • Difference between HPLC and UPLC​

  • Components of an HPLC instrument including columns, detectors, pumps etc.​

  • Types of HPLC techniques including reverse and normal phase HPLC, affinity chromatography etc.​

  • Components of a gas chromatograph and their function​

  • Usage of carrier gases (mobile phase), columns (stationary phase), and detectors​

  • Graded assessment​

  • Why it matters

    Handling of instruments by untrained personnel can lead to erroneous and non-reproducible results, leading to OOS/OOT and further time loss in root cause investigation.. So it is important to understand the science and theories behind chromatography techniques to avoid mistakes, and to identify the correct root cause to avoid repeat errors.​

  • Who is it for​ ​

    This module is designed for all employees in analytical quality control and analytical quality assurance, as well as in teams involved in performing and reviewing of chromatographic methods.​

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