A Practical Guide to Sample Preparation Techniques - Part 2
Part 2 of this two-part module covers the basics of sample preparation focusing on pipetting, filtration, centrifugation and vial filling.
Principle of pipetting, graduated pipettes, and pipetting parameters
Selection of the right pipette and reading from a pipette (meniscus)
Pipetting steps and critical precautions
Introduction to filtration and types of filters
Syringe filters and steps to filtration
Critical parameters and precautions
Introduction to centrifugation and types of centrifuges
Difference between centrifugation and filtration
Centrifugation steps and the critical parameters
Vial filling using syringe filtration
Vial labeling and crimping
Typical do’s and don’ts for all techniques
Why it matters
Proper sample preparation is critical to ensure that evaluation of batch quality is accurately performed, and to ensure reproducible results. Pipetting is used to measure and transfer an accurate volume of liquid. Centrifugation and filtration are different ways of removing solids from liquids and ensuring that the samples used for analysis are particle-free. Understanding and training on these techniques will ensure exact sample preparation and accurate results.
Who is it for
This module is for employees in production quality control and quality assurance who prepare samples for in-process or final testing.